Interaction of guanosine nucleotides with elongation factor 2. II. Effect of ribosomes and magnesium ions on guanosine diphosphate and guanosine triphosphate binding to the enzyme
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Interaction of guanosine nucleotides with elongation factor 2. II. Effect of ribosomes and magnesium ions on guanosine diphosphate and guanosine triphosphate binding to the enzyme.
The effects of ribosomes and Mg-2plus on the binding of GDP and GTP to elongation factor 2 (EF-2) have been studied by an improved filter-binding assay. Both ribosomes and Mg-2plus strongly inhibit the binding of GDP but have apparently no effect on the GTP binding to the enzyme. An apparent stimulation by ribosomes of GTP binding to EF-2 is time-dependent and parallels a concomitant increase o...
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Chaykin, S., Law, J., Phillips, A. H., Tchen, T. T. & Bloch, K. (1958). Proc. nat. Acad. Sci., Wash., 44, 998. Cornforth, J. W., Cornforth, R. H., Popjak, G. & Gore, I. Y. (1958). Biochem. J. 69, 146. de Waard, A. (1958). Doctoral Thesis: University of Utrecht, Holland; Investigations on the intermediates in the biosynthesis of fatty acids and cholesterol. Nijmegen: Drukkerij Gebr. Janssen. de ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1975
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)41956-x